Phosphatidic acid is a major phospholipid class in reproductive organs of Arabidopsis thaliana

Plant Signal Behav. 2015;10(8):e1049790. doi: 10.1080/15592324.2015.1049790.


Phospholipids are the crucial components of biological membranes and signal transduction. Among different tissues, flower phospholipids are one of the least characterized features of plant lipidome. Here, we report that floral reproductive organs of Arabidopsis thaliana contain high levels of phosphatidic acid (PA), a known lipid second messenger. By using floral homeotic mutants enriched with specific floral organs, lipidomics study showed increased levels of PA species in ap3-3 mutant with enriched pistils. Accompanied gene expression study for 7 diacylglycerol kinases and 11 PA phosphatases revealed distinct floral organ specificity, suggesting an active phosphorylation/dephosphorylation between PA and diacylglycerol in flowers. Our results suggest that PA is a major phospholipid class in floral reproductive organs of A. thaliana.

Keywords: Arabidopsis thaliana; floral reproductive organs; glycerolipids; homeotic mutants; phosphatidic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Diacylglycerol Kinase / metabolism
  • Diglycerides / metabolism
  • Flowers / metabolism*
  • Gene Expression
  • Gene Expression Regulation, Plant
  • Homeodomain Proteins / metabolism
  • MADS Domain Proteins / genetics
  • MADS Domain Proteins / metabolism
  • Meristem / metabolism*
  • Mutation
  • Phosphatidic Acids / metabolism*
  • Phospholipids / metabolism
  • Phosphoric Monoester Hydrolases / metabolism
  • Phosphorylation


  • APETALA 3 protein, Arabidopsis
  • Arabidopsis Proteins
  • Diglycerides
  • Homeodomain Proteins
  • MADS Domain Proteins
  • Phosphatidic Acids
  • Phospholipids
  • Diacylglycerol Kinase
  • Phosphoric Monoester Hydrolases