Development of a rapid, reliable and quantitative method--"SPOTi" for testing antifungal efficacy

Khalida Rizi; Sudaxshina Murdan; Cynthia A Danquah; Jane Faull; Sanjib Bhakta

doi:10.1016/j.mimet.2015.07.005

Development of a rapid, reliable and quantitative method--"SPOTi" for testing antifungal efficacy

J Microbiol Methods. 2015 Oct:117:36-40. doi: 10.1016/j.mimet.2015.07.005. Epub 2015 Jul 13.

Authors

Khalida Rizi¹, Sudaxshina Murdan², Cynthia A Danquah³, Jane Faull³, Sanjib Bhakta⁴

Affiliations

¹ Department of Pharmaceutics, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK; Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK.
² Department of Pharmaceutics, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK.
³ Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK.
⁴ Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK. Electronic address: s.bhakta@bbk.ac.uk.

PMID: 26183763
DOI: 10.1016/j.mimet.2015.07.005

Abstract

A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the anti-tubercular drug discovery field. The fungal-SPOTi assay correlated with the disc-diffusion method, and is validated using mycelial plugs. We propose the fungal-SPOTi as a high-throughput alternative to the disc-diffusion and broth micro-dilution anti-fungal assays to screen novel anti-fungals.

Keywords: Antifungals; Dermatophytes; Drug discovery; SPOTi; Screening.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antifungal Agents / pharmacology*
Arthrodermataceae / drug effects
Drug Discovery / methods*
High-Throughput Screening Assays / methods*
Microbial Sensitivity Tests / methods*
Trichophyton / drug effects

Substances

Antifungal Agents