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Comment
. 2015 Jul 16;59(2):146-8.
doi: 10.1016/j.molcel.2015.07.002.

Putting Non-coding RNA on Display With CRISPR

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Putting Non-coding RNA on Display With CRISPR

Pablo Perez-Pinera et al. Mol Cell. .
Free PMC article

Abstract

In a recent issue of Nature Methods, Shechner et al. (2015) reported the development of CRISPR Display (CRISP-Disp), which is a sophisticated, flexible, modular, and multiplexable platform for targeting different types of non-coding RNAs (ncRNAs) to genomic loci. CRISP-Disp will facilitate synthetic-biology applications and enable the elucidation of ncRNA functions.

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Figure 1.
Figure 1.. Structure and Applications of CRISP-Disp
(A and B) Schematic representation of two CRISPR systems for gene activation. Catalytically inactive Cas9 (dCas9) is targeted to a particular locus by a short RNA sequence known as a guide RNA (gRNA). The VP64 transcriptional activation domain can be recruited to the complex by fusion to dCas9 (direct activation, A) or by fusion to RNA-binding proteins that bind to the gRNA (bridged activation, B).Shechner et al. (2015) demonstrated that dCas9 can be targeted to genomic DNA loci by modified gRNAs that are part of larger RNA cargoes. This system, named CRISP-Disp, enables ncRNA targeting for studies that require ncRNA recruitment and functionalization, natural RNA-binding protein recruitment, or affinity tagging (C).

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