The evaluation of the healing proprieties of pumpkin and linseed oils on deep second-degree burns in rats

Pharm Biol. 2016;54(4):581-7. doi: 10.3109/13880209.2015.1067233. Epub 2015 Jul 17.


Context: Medicinal plants have been recognized as useful remedies for primary health care. Accordingly, Cucurbita pepo L. (Cucurbitaceae) (pumpkin) and Linum usitatissimum (L.) Griesb. (Linaceae) (linseed) which have extracted oil with prominent pharmacological properties are investigated as possible burn healing treatments.

Objective: The present study assesses the healing potential of pumpkin and linseed extracted oils on rats.

Materials and methods: Uniform deep second-degree burns were induced on the dorsum of 24 rats, randomly divided into four groups. The burns were measured, photographed, and topically treated with saline solution, "Cytol Centella®", pumpkin, and linseed-extracted oils (0.52 µl/mm(2) of oil) each 2 d (up until day 33). Post-burning of the 33rd day, biopsies were histologically assessed.

Results: At the end of the experiment, the rat groups treated with linseed, pumpkin oils, and "Cytol Centella®" had higher percentage of wound contraction (98.68, 96.71, and 92.54%, respectively) than the control group (58.38%). Wound biopsies from rats treated with extracted oils showed the best tissue regeneration proprieties as compared with the other groups. The histomorphometric analysis of biopsies revealed that linseed oil could significantly stimulate angiogenesis (55.6% ± 7.25). The pumpkin oil, and Cytol Centella® could significantly increase the collagen production 64.9% ± 5.94, and 61.2% ± 7.36, respectively.

Discussion and conclusion: Overall, our study has given for the first time scientific evidence of the healing efficiency of pumpkin and linseed oils on burn-wounds.

Keywords: Angiogenesis; collagen; wound healing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Burns / drug therapy*
  • Burns / pathology
  • Cucurbita*
  • Drug Evaluation, Preclinical / methods
  • Flax*
  • Linseed Oil / isolation & purification
  • Linseed Oil / pharmacology
  • Linseed Oil / therapeutic use*
  • Male
  • Plant Oils / isolation & purification
  • Plant Oils / pharmacology
  • Plant Oils / therapeutic use
  • Rats
  • Rats, Wistar
  • Seeds
  • Wound Healing / drug effects*
  • Wound Healing / physiology


  • Plant Oils
  • Linseed Oil