A Complex Signaling Cascade Governs Pristinamycin Biosynthesis in Streptomyces pristinaespiralis

Yvonne Mast; Jamil Guezguez; Franziska Handel; Eva Schinko

doi:10.1128/AEM.00728-15

A Complex Signaling Cascade Governs Pristinamycin Biosynthesis in Streptomyces pristinaespiralis

Appl Environ Microbiol. 2015 Oct;81(19):6621-36. doi: 10.1128/AEM.00728-15. Epub 2015 Jul 17.

Authors

Yvonne Mast¹, Jamil Guezguez², Franziska Handel², Eva Schinko²

Affiliations

¹ Department of Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, Faculty of Science, University of Tübingen, Tübingen, Germany yvonne.mast@biotech.uni-tuebingen.de.
² Department of Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, Faculty of Science, University of Tübingen, Tübingen, Germany.

Abstract

Pristinamycin production in Streptomyces pristinaespiralis Pr11 is tightly regulated by an interplay between different repressors and activators. A γ-butyrolactone receptor gene (spbR), two TetR repressor genes (papR3 and papR5), three SARP (Streptomyces antibiotic regulatory protein) genes (papR1, papR2, and papR4), and a response regulator gene (papR6) are carried on the large 210-kb pristinamycin biosynthetic gene region of Streptomyces pristinaespiralis Pr11. A detailed investigation of all pristinamycin regulators revealed insight into a complex signaling cascade, which is responsible for the fine-tuned regulation of pristinamycin production in S. pristinaespiralis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Gene Expression Regulation, Bacterial*
Pristinamycin / biosynthesis*
Streptomyces / genetics
Streptomyces / metabolism*
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

Bacterial Proteins
Pristinamycin
Transcription Factors