Nanogram nitrite and nitrate determination in environmental and biological materials by vanadium (III) reduction with chemiluminescence detection

Anal Chem. 1989 Dec 15;61(24):2715-8. doi: 10.1021/ac00199a007.


Nitrite in environmental water samples is reduced at room temperature to nitric oxide in acidic medium containing vanadium (III). Nitrate is also rapidly reduced after heating to 80-90 degrees C. Nitric oxide is removed from the reaction solution by scrubbing with helium carrier gas and is detected by means of a chemiluminescence NOx analyzer. Nanogram detection limits are obtained. The method has the advantage of not requiring highly acidic solutions for nitrate reduction and has been applied to the analysis of a variety of environmental waters, sediment, plant materials, and human urine and blood serum.

MeSH terms

  • Body Fluids / analysis*
  • Humans
  • Luminescent Measurements
  • Nitrates / analysis*
  • Nitrites / analysis*
  • Oxidation-Reduction
  • Plants / analysis
  • Vanadium
  • Water / analysis*


  • Nitrates
  • Nitrites
  • Vanadium
  • Water