Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 May 1;8(5):5363-70.
eCollection 2015.

Amygdalin-mediated Inhibition of Non-Small Cell Lung Cancer Cell Invasion in Vitro

Affiliations
Free PMC article

Amygdalin-mediated Inhibition of Non-Small Cell Lung Cancer Cell Invasion in Vitro

Liyu Qian et al. Int J Clin Exp Pathol. .
Free PMC article

Abstract

Lung cancer is a common malignant tumor claiming the highest fatality worldwide for a long period of time. Unfortunately, most of the current treatment methods are still based on the characteristics of cancer cells in the primary lesion and the prognosis is often much poorer in patients with metastatic cancers. Amygdalin, a natural product of glycosides and lots of evidence shows that amygdalin can inhibit the proliferation of some kinds of cancer cells. In this study, we first obtained the highly metastatic NSCLC cell lines H1299/M and PA/M and further treated these cells with amygdalin. We found that the in vitro proliferability of H1299/M and PA/M was inhibited, but such inhibition required higher concentration of amygdalin. When lower concentration of amygdalin was used for the experiments, we observed that the in vitro invasive and migration capacities of H1299/M and PA/M were significantly inhibited. These results strongly suggested that amygdalin was likely to have anti-metastatic NSCLC effect. This study offers information of the role of amygdalin that may be useful as a therapeutic target in lung tumors.

Keywords: Lung cancer; amygdalin; invasion.

Figures

Figure 1
Figure 1
Enhanced metastatic ability of H1299/M and PA/M cell lines. A. Representative images of wound-healing assay for H1299/M cell migration. B. Representative images of transwell assay for H1299/M cell invasion. C. Representative images of wound-healing assay for PA/M cell migration. D. Representative images of transwell assay for PA/M cell invasion. E. The relative metastasis of H1299/M and PA/M. *P<0.05; **P<0.01, compared with the control. F. Effect of amygdalin on the proliferation of H1299/M cells as assessed by MTT assay.
Figure 2
Figure 2
Effects of amygdalin on the metastatic capacities of H1299/M and PA/M cell lines. A. Representative wound-healing assay images for H1299/M cell migration pretreatment with different amygdalin concentration. B. Representative transwell assay images for H1299/M cell invasion pretreatment with different amygdalin concentration. C. Representative wound-healing assay images for PA/M cell migration pretreatment with different amygdalin concentration. D. Representative transwell assay images for PA/M cell invasion pretreatment with different amygdalin concentration. E/F. The relative cells invasion and migration of H1299/M and PA/M cells treatment with different amygdalin concentration. *P<0.05; **P<0.01, compared with the control.
Figure 3
Figure 3
Effects of amygdalin on cell signaling pathway in H1299/M and PA/M cell lines. A/D. The mRNA expressions of integrin β1, integrin β4, ILK, FAK, β-catenin, MMP2, MMP9, CD44 and E-cadherin in H1299/M and PA/M cells pretreatment with amygdalin were analyzed by real-time PCR. Real-time PCR reactions were performed on templates of cDNA from H1299/M and PA/M cell lines using a set of primers as in Materials and methods. *P<0.05; ** P<0.01, compared with the control. B/C. The protein expressions of integrin β1, integrin β4, ILK, FAK, p-FAK, MMP2, MMP9, CD44 and E-cadherin in H1299/M and PA/M cells pretreatment with amygdalin were analyzed by Western blot. E/F. The p-Akt and p-RICTOR protein expressions in H1299/M and PA/M cells were analyzed by Western blot.

Similar articles

See all similar articles

Cited by 9 articles

See all "Cited by" articles

MeSH terms

Substances

Feedback