Evaluation of the potential anti-allergic effects of heat-inactivated Lactobacillus paracasei V0151 in vitro, ex vivo, and in vivo

Benef Microbes. 2015;6(5):697-705. doi: 10.3920/BM2014.0159. Epub 2015 Jul 20.


The efficacy of Lactobacillus paracasei V0151 (V0151), isolated from the faeces of a child, to modulate immune responses was investigated. In RAW 264.7 cells expressing an inducible nitric oxide synthase (iNOS)-directed luciferase gene, heat-inactivated V0151 stimulated iNOS expression followed by nitric oxide production. V0151 significantly elevated interferon gamma, interleukin (IL)-10, tumour necrosis factor alpha, and IL-1β production in human peripheral blood mononuclear cells. In splenocytes isolated from ovalbumin (OVA)-sensitised BALB/c mice treated with OVA and V0151 at different bacterium-to-cell ratios (1:1, 10:1, and 20:1) for 96 h, IL-2, IL-4, IL-5, and IL-13 production was dose-dependently downregulated, whereas IL-12 was dose-dependently upregulated. Collectively, our findings indicate that V0151 might regulate pro-inflammatory factors in macrophages and splenocytes. Furthermore, the T helper 1/T helper 2 (Th1/Th2) balance was also skewed toward Th1 dominance through the elevation of Th1 cytokine production.

Keywords: Lactobacillus paracasei; immune modulation; macrophages; ovalbumin; splenocytes.

MeSH terms

  • Animals
  • Anti-Allergic Agents / pharmacology*
  • Cells, Cultured
  • Cytokines / metabolism
  • Hot Temperature*
  • Lactobacillus / immunology*
  • Lactobacillus / radiation effects*
  • Leukocytes, Mononuclear / immunology
  • Macrophages / immunology
  • Mice, Inbred BALB C
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase Type II / biosynthesis


  • Anti-Allergic Agents
  • Cytokines
  • Nitric Oxide
  • Nitric Oxide Synthase Type II