Isolation and quantitation of Clostridium difficile in aqueous and fecal matter using two types of selective media

Seth T Housman; Mary Anne Banevicius; Lucinda M Lamb; David P Nicolau

doi:10.1016/j.jmii.2015.05.012

Isolation and quantitation of Clostridium difficile in aqueous and fecal matter using two types of selective media

J Microbiol Immunol Infect. 2016 Jun;49(3):445-7. doi: 10.1016/j.jmii.2015.05.012. Epub 2015 Jun 27.

Authors

Seth T Housman¹, Mary Anne Banevicius¹, Lucinda M Lamb¹, David P Nicolau²

Affiliations

¹ Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT, USA.
² Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT, USA; Division of Infectious Diseases, Hartford Hospital, Hartford, CT, USA. Electronic address: david.nicolau@hhchealth.org.

PMID: 26198865
DOI: 10.1016/j.jmii.2015.05.012

Abstract

We evaluated the isolation and quantitation of Clostridium difficile from aqueous and fecal samples utilizing ChromID CDIF and cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate media. Growth was similar between the two. ChromID CDIF provided enhanced isolation and required no ethanol pretreatment to inhibit normal flora. ChromID CDIF also improved turn-around time, requiring only 24 hours' incubation.

Keywords: Chromogenic agar; Clostridium difficile; Cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate.

Publication types

Evaluation Study

MeSH terms

Bacteriological Techniques / methods*
Cefoxitin / pharmacology
Chromogenic Compounds / pharmacology
Clostridioides difficile / growth & development*
Clostridioides difficile / isolation & purification*
Culture Media
Cycloserine / pharmacology
Enterocolitis, Pseudomembranous / diagnosis*
Enterocolitis, Pseudomembranous / microbiology
Feces / microbiology*
Fructose / metabolism
Humans

Substances

Chromogenic Compounds
Culture Media
Fructose
Cefoxitin
Cycloserine