Lipopolysaccharide binding protein is an adipokine involved in the resilience of the mouse adipocyte to inflammation

Diabetologia. 2015 Oct;58(10):2424-34. doi: 10.1007/s00125-015-3692-7. Epub 2015 Jul 23.


Aims/hypothesis: Lipopolysaccharide (LPS) binding protein (LBP) is a novel 65 kDa adipokine, linked to adipose tissue (AT) inflammation, obesity and insulin resistance, that inhibits adipocyte differentiation. Here, we investigated the molecular mechanisms behind these detrimental effects on adipogenesis through whole-genome transcriptomics and in vitro experiments.

Methods: Permanent and transient knockdown (KD) and co-culture experiments were performed in 3T3-L1 and 3T3-F442A cell lines during adipocyte differentiation. Microarray gene expression was performed using Genechip Affymetrix technology and validated by real-time PCR.

Results: LBP KD of 3T3-L1 cells led to a potentiated adipocyte differentiation with a dose-response relationship; genes involved in mitochondrial biogenesis, fatty acid metabolism and peroxisome proliferator-activated receptor γ (PPAR-γ) action were dramatically upregulated in parallel to increased insulin signalling. Cells with LBP KD became refractory to proinflammatory cytokines and other inflammatory stimuli (LPS and palmitate). This phenotype, mediated through disrupted nuclear factor κB (NFκB) signalling, was reversed by a soluble factor present in a co-culture with native cells and by exogenous LBP. Double-silencing of LBP and toll-like receptor 4 (TLR4) again rendered these cells insensitive to co-culture, LBP and inflammatory factors.

Conclusions/interpretation: In summary, LBP is a proinflammatory soluble adipokine that acts as a brake for adipogenesis, strengthening the negative effects of palmitate and LPS on adipocyte differentiation.

Keywords: Adipogenesis; Inflammation; LPS; Lipopolysaccharide binding protein; Palmitate; TLR4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Acute-Phase Proteins / genetics
  • Acute-Phase Proteins / metabolism*
  • Adipocytes / drug effects
  • Adipocytes / metabolism*
  • Adipogenesis / drug effects
  • Adipogenesis / genetics*
  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Gene Expression
  • Gene Knockdown Techniques
  • Inflammation / genetics
  • Inflammation / metabolism*
  • Lipopolysaccharides / pharmacology
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism*
  • Mice
  • NF-kappa B / metabolism
  • Palmitic Acid / pharmacology
  • Signal Transduction / drug effects
  • Signal Transduction / genetics


  • Acute-Phase Proteins
  • Carrier Proteins
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • NF-kappa B
  • lipopolysaccharide-binding protein
  • Palmitic Acid