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Review
, 89 (19), 9706-8

Going Viral With Fluorescent Proteins

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Review

Going Viral With Fluorescent Proteins

Lindsey M Costantini et al. J Virol.

Abstract

Many longstanding questions about dynamics of virus-cell interactions can be answered by combining fluorescence imaging techniques with fluorescent protein (FP) tagging strategies. Successfully creating a FP fusion with a cellular or viral protein of interest first requires selecting the appropriate FP. However, while viral architecture and cellular localization often dictate the suitability of a FP, a FP's chemical and physical properties must also be considered. Here, we discuss the challenges of and offer suggestions for identifying the optimal FPs for studying the cell biology of viruses.

Figures

FIG 1
FIG 1
Size comparison of GFP with viral proteins illustrates the potential effect of steric hindrance. (A) Size comparison of GFP (PDB code 2B3P), the coat protein of tobacco mosaic virus (2OM3), and 1918 influenza virus hemagglutinin membrane glycoprotein (1RUZ) relative to 5-nm scale bar. (B) Hypothetical fusion protein of a major capsid protein, Vp54 of paramecium bursaria chlorella virus type 1 (1M4X), with GFP. The trimeric capsomer and associated GFP molecules represent the potential restrictions of a viral FP fusion protein.

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