Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 99 (3), 470-83

Nucleoside Uptake in Vibrio Cholerae and Its Role in the Transition Fitness From Host to Environment

Affiliations

Nucleoside Uptake in Vibrio Cholerae and Its Role in the Transition Fitness From Host to Environment

Tanja Gumpenberger et al. Mol Microbiol.

Abstract

As it became evident recently, extracellular DNA could be a versatile nutrient source of the facultative pathogen Vibrio cholerae along the different stages of its life cycle. By the use of two extracellular nucleases and periplasmic phosphatases, V. cholerae degrades extracellular DNA to nucleosides. In this study, we investigated the nucleoside uptake via identification and characterization of VCA0179, VC1953 and VC2352 representing the three nucleoside transport systems in V. cholerae. Based on our results VC2352 seems to be the dominant nucleoside transporter. Nevertheless, all three transporters are functional and can contribute to the utilization of nucleosides as a sole source of carbon or nitrogen. We found that the transcriptional activity of these three distal genes is equally promoted or antagonized by CRP or CytR respectively. Finally, mutants impaired for nucleoside uptake exhibit decreased transition fitness from the host into low carbon environments along the life cycle of V. cholerae.

Similar articles

See all similar articles

Cited by 5 PubMed Central articles

Publication types

MeSH terms

LinkOut - more resources

Feedback