Neural differentiation of Xenopus laevis ectoderm takes place after disaggregation and delayed reaggregation without inducer

Cell Differ Dev. 1989 Dec;28(3):211-7. doi: 10.1016/0922-3371(89)90006-3.


When Xenopus blastula or early gastrula ectoderm is disaggregated and cells are kept dispersed for up to 5 h prior to reaggregation, the resulting spheres will differentiate into large neural structures. In contrast, dissociated and immediately reaggregated ectoderm will only differentiate into ciliated epidermis (so-called 'atypical epidermis'). Ectoderm treated with mesoderm-inducing XTC-conditioned medium during the period of reaggregation immediately after disaggregation will only form one- or two-cell types (notochord and somites) only. Ectoderm treated with XTC-factor prior to disaggregation will differentiate into a large variety of cell types.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Aggregation / drug effects
  • Cell Differentiation / drug effects
  • Ectoderm / cytology*
  • Ectoderm / drug effects
  • Female
  • Mesoderm / physiology
  • Nervous System / cytology
  • Nervous System / drug effects
  • Nervous System / embryology
  • Neurons / cytology*
  • Neurons / drug effects
  • Xenopus laevis / embryology*