Quantitative analysis of the Escherichia coli proteome

Jacek R Wiśniewski; Dariusz Rakus

doi:10.1016/j.dib.2014.08.004

Quantitative analysis of the Escherichia coli proteome

Data Brief. 2014 Aug 22:1:7-11. doi: 10.1016/j.dib.2014.08.004. eCollection 2014 Dec.

Authors

Jacek R Wiśniewski¹, Dariusz Rakus²

Affiliations

¹ Biochemical Proteomics Group, Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
² Department of Animal Molecular Physiology, Wroclaw University, Wroclaw, Poland.

Abstract

Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC-MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quantitation was performed using the 'Total Protein Approach'. The data are supplied in the article.

Keywords: Absolute protein quantification; Escherichia coli proteome; Filter-aided sample preparation, FASP; Protein copy number; Total protein approach.