Quantitative analysis of the myelin g-ratio from electron microscopy images of the macaque corpus callosum

Nikola Stikov; Jennifer S W Campbell; Thomas Stroh; Mariette Lavelée; Stephen Frey; Jennifer Novek; Stephen Nuara; Ming-Kai Ho; Barry J Bedell; Robert F Dougherty; Ilana R Leppert; Mathieu Boudreau; Sridar Narayanan; Tanguy Duval; Julien Cohen-Adad; Paul-Alexandre Picard; Alicja Gasecka; Daniel Côté; G Bruce Pike

doi:10.1016/j.dib.2015.05.019

Quantitative analysis of the myelin g-ratio from electron microscopy images of the macaque corpus callosum

Data Brief. 2015 Jun 17:4:368-73. doi: 10.1016/j.dib.2015.05.019. eCollection 2015 Sep.

Authors

Nikola Stikov¹, Jennifer S W Campbell², Thomas Stroh², Mariette Lavelée², Stephen Frey², Jennifer Novek², Stephen Nuara², Ming-Kai Ho², Barry J Bedell², Robert F Dougherty³, Ilana R Leppert², Mathieu Boudreau², Sridar Narayanan², Tanguy Duval⁴, Julien Cohen-Adad⁴, Paul-Alexandre Picard⁵, Alicja Gasecka⁶, Daniel Côté⁶, G Bruce Pike⁷

Affiliations

¹ Montreal Neurological Institute, McGill University, Montreal, Canada ; École Polytechnique de Montréal, Montréal, Canada.
² Montreal Neurological Institute, McGill University, Montreal, Canada.
³ Stanford University, Stanford, CA, United States.
⁴ École Polytechnique de Montréal, Montréal, Canada.
⁵ École supérieure d׳ingénieurs en génie électrique, Rouen, France.
⁶ Université Laval, Quebec City, Canada.
⁷ Montreal Neurological Institute, McGill University, Montreal, Canada ; Hotchkiss Brain Institute and Department of Radiology, University of Calgary, Calgary, Alberta, Canada.

Abstract

We provide a detailed morphometric analysis of eight transmission electron micrographs (TEMs) obtained from the corpus callosum of one cynomolgus macaque. The raw TEM images are included in the article, along with the distributions of the axon caliber and the myelin g-ratio in each image. The distributions are analyzed to determine the relationship between axon caliber and g-ratio, and compared against the aggregate metrics (myelin volume fraction, fiber volume fraction, and the aggregate g-ratio), as defined in the accompanying research article entitled 'In vivo histology of the myelin g-ratio with magnetic resonance imaging' (Stikov et al., NeuroImage, 2015).