Bioactivity characterization of Lactobacillus strains isolated from dairy products

Microbiologyopen. 2015 Oct;4(5):803-13. doi: 10.1002/mbo3.280. Epub 2015 Jul 27.


This study aimed to find candidate strains of Lactobacillus isolated from sheep dairy products (yogurt and ewe colostrum) with probiotic and anticancer activity. A total of 100 samples were randomly collected from yogurt and colostrum and 125 lactic acid bacteria were isolated. Of these, 17 Lactobacillus strains belonging to five species (L. delbrueckii, L. plantarum, L. rhamnosus, L. paracasei, and L. casei) were identified. L. plantarum 17C and 13C, which isolated from colostrums, demonstrated remarkable results such as resistant to low pH and high concentrations of bile salts, susceptible to some antibiotics and good antimicrobial activity that candidate them as potential probiotics. Seven strains (1C, 5C, 12C, 13C, 17C, 7M, and 40M), the most resistant to simulated digestion, were further investigated to evaluate their capability to adhere to human intestinal Caco-2 cells. L. plantarum 17C was the most adherent strain. The bioactivity assessment of L. plantarum 17C showed anticancer effects via the induction of apoptosis on HT-29 human cancer cells and negligible side effects on one human epithelial normal cell line (FHs 74). The metabolites produced by this strain can be used as alternative pharmaceutical compounds with promising therapeutic indices because they are not cytotoxic to normal mammalian cells.

Keywords: Colostrum; Lactobacillus; molecular method; probiotic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Antibiosis
  • Antineoplastic Agents / isolation & purification*
  • Antineoplastic Agents / pharmacology*
  • Apoptosis
  • Bacterial Adhesion
  • Bile Acids and Salts / toxicity
  • Cell Line
  • Colostrum / microbiology
  • Dairy Products / microbiology*
  • Epithelial Cells / microbiology
  • Epithelial Cells / physiology
  • Humans
  • Hydrogen-Ion Concentration
  • Lactobacillus / classification
  • Lactobacillus / drug effects
  • Lactobacillus / isolation & purification*
  • Lactobacillus / physiology*
  • Probiotics / isolation & purification*
  • Probiotics / pharmacology*
  • Sheep


  • Anti-Bacterial Agents
  • Antineoplastic Agents
  • Bile Acids and Salts