Structural Determinants for Substrate Binding and Catalysis in Triphosphate Tunnel Metalloenzymes

J Biol Chem. 2015 Sep 18;290(38):23348-60. doi: 10.1074/jbc.M115.674473. Epub 2015 Jul 28.

Abstract

Triphosphate tunnel metalloenzymes (TTMs) are present in all kingdoms of life and catalyze diverse enzymatic reactions such as mRNA capping, the cyclization of adenosine triphosphate, the hydrolysis of thiamine triphosphate, and the synthesis and breakdown of inorganic polyphosphates. TTMs have an unusual tunnel domain fold that harbors substrate- and metal co-factor binding sites. It is presently poorly understood how TTMs specifically sense different triphosphate-containing substrates and how catalysis occurs in the tunnel center. Here we describe substrate-bound structures of inorganic polyphosphatases from Arabidopsis and Escherichia coli, which reveal an unorthodox yet conserved mode of triphosphate and metal co-factor binding. We identify two metal binding sites in these enzymes, with one co-factor involved in substrate coordination and the other in catalysis. Structural comparisons with a substrate- and product-bound mammalian thiamine triphosphatase and with previously reported structures of mRNA capping enzymes, adenylate cyclases, and polyphosphate polymerases suggest that directionality of substrate binding defines TTM catalytic activity. Our work provides insight into the evolution and functional diversification of an ancient enzyme family.

Keywords: adenylate cyclase (adenylyl cyclase); enzyme mechanism; inorganic polyphosphatase; manganese; nuclear magnetic resonance (NMR); protein structure; thiamine triphosphatase; x-ray crystallography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis Proteins / chemistry*
  • Arabidopsis Proteins / genetics
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Escherichia coli Proteins / chemistry*
  • Escherichia coli Proteins / genetics
  • Evolution, Molecular
  • Inorganic Pyrophosphatase / chemistry*
  • Inorganic Pyrophosphatase / genetics
  • Metalloproteins / chemistry*
  • Metalloproteins / genetics
  • Structural Homology, Protein

Substances

  • Arabidopsis Proteins
  • Escherichia coli Proteins
  • Metalloproteins
  • Inorganic Pyrophosphatase

Associated data

  • PDB/1D8H
  • PDB/3BHD
  • PDB/3G3R
  • PDB/3N0Y
  • PDB/3N10
  • PDB/3v85
  • PDB/5a5y
  • PDB/5a60
  • PDB/5a61
  • PDB/5a64
  • PDB/5a65
  • PDB/5a66
  • PDB/5a67
  • PDB/5a68