Anti-inflammatory and anti-arthritic activity of aqueous extract of Rosa centifolia in experimental rat models

Int J Rheum Dis. 2017 Sep;20(9):1072-1078. doi: 10.1111/1756-185X.12625. Epub 2015 Jul 27.


Aim: The present study was carried out to evaluate the anti-inflammatory and antiarthritic activity of Rosa centifolia aqueous extract (RC) in a carrageenan-induced paw edema model and complete Freund's adjuvant (CFA)-induced arthritis.

Methods: Anti-inflammatory activity of RC was evaluated using the carrageenan-induced paw edema model in rats. Arthritis was induced in rats by sub-plantar administration of CFA. Joint size was measured at regular intervals by using a micrometer screw gauge. Serum and ankle joints of rats immunized with CFA were collected and subjected to enzyme-linked immunosorbent assay for estimation of tumor necrosis factor (TNF)-α level and dot blot for secretory cytokines interleukin (IL)-1β and IL-6. An acute and 28-day oral toxicity study was carried out to evaluate the safety of the test drug.

Results: Pre-treatment with RC produced a dose-dependent reduction in carrageenan-induced paw edema and CFA-induced arthritis models and was effective as indomethacin. RC also inhibited the delayed increase in joint diameter as seen in control and indomethacin-treated animals in CFA-induced arthritis. The expression of proinflammatory mediators TNF-α, IL-6 and IL-1β was also found to be less in the RC-treated group as compared to controls.

Conclusion: Based on these results, it was suggested that Rosa centifolia could be considered as a potential anti-inflammatory and anti-arthritic agent.

Keywords: Rosa centifolia; adjuvant arthritis; carrageenan; inflammation; rheumatoid arthritis.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology*
  • Anti-Inflammatory Agents / toxicity
  • Antirheumatic Agents / isolation & purification
  • Antirheumatic Agents / pharmacology*
  • Antirheumatic Agents / toxicity
  • Arthritis, Experimental / blood
  • Arthritis, Experimental / chemically induced
  • Arthritis, Experimental / pathology
  • Arthritis, Experimental / prevention & control*
  • Carrageenan
  • Dose-Response Relationship, Drug
  • Edema / chemically induced
  • Edema / prevention & control*
  • Freund's Adjuvant
  • Indomethacin / pharmacology
  • Inflammation Mediators / blood
  • Interleukin-1beta / blood
  • Interleukin-6 / blood
  • Joints / drug effects*
  • Joints / metabolism
  • Joints / pathology
  • Male
  • Phytotherapy
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plant Extracts / toxicity
  • Plants, Medicinal
  • Rats, Wistar
  • Rosa* / chemistry
  • Time Factors
  • Tumor Necrosis Factor-alpha / blood


  • Anti-Inflammatory Agents
  • Antirheumatic Agents
  • IL1B protein, rat
  • Inflammation Mediators
  • Interleukin-1beta
  • Interleukin-6
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • Carrageenan
  • Freund's Adjuvant
  • Indomethacin