Density gradients at hydrogel interfaces for enhanced cell penetration

Biomater Sci. 2015 Apr;3(4):586-91. doi: 10.1039/c4bm00416g. Epub 2015 Jan 14.


We report that stiffness gradients facilitate infiltration of cells through otherwise cell-impermeable hydrogel interfaces. By enabling the separation of hydrogel manufacturing and cell seeding, and by improving cell colonization of additively manufactured hydrogel elements, interfacial density gradients present a promising strategy to progress in the creation of 3D tissue models.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocompatible Materials / chemistry*
  • Cell Adhesion / drug effects
  • Cell Culture Techniques
  • Hydrogels / chemistry*


  • Biocompatible Materials
  • Hydrogels