Structural and functional changes in high-density lipoprotein induced by chemical modification

Biomater Sci. 2015 May;3(5):712-5. doi: 10.1039/c4bm00402g. Epub 2015 Mar 5.


Reconstituted high-density lipoprotein (rHDL), a natural nanoparticle consisting of apolipoprotein A-I and phospholipids, was modified with a hydrophobic fluorescent dye before (pre-rHDL) and after (post-rHDL) reconstitution. Pre-rHDL particles had a similar size to unmodified rHDL, but post-rHDL particles were significantly larger and their avidity for a HDL receptor was 2.6 times of that shown by pre-rHDL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apolipoprotein A-I / chemistry*
  • Apolipoprotein A-I / metabolism
  • Cholesterol, VLDL / chemistry*
  • Cholesterol, VLDL / metabolism
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Lipoproteins, HDL / chemistry*
  • Lipoproteins, HDL / metabolism
  • Micelles
  • Nanoparticles / chemistry*
  • Phospholipids / chemistry


  • Apolipoprotein A-I
  • Cholesterol, VLDL
  • Lipoproteins, HDL
  • Micelles
  • Phospholipids