Association of the leptin receptor Gln223 Arg polymorphism with lipid profile in obese Pakistani subjects

Nutrition. 2015 Sep;31(9):1136-40. doi: 10.1016/j.nut.2015.05.001. Epub 2015 May 29.


Objective: Leptin receptor gene (LEPR) encodes a receptor for leptin hormone expressed in hypothalamus and single nucleotide polymorphisms in LEPR may have a considerable effect on obesity phenotype. The aim of this study was to investigate an extensively studied LEPR polymorphism Gln223 Arg in a Pakistani study group and its association patterns with different serum biochemical parameters.

Methods: The study included 250 obese and 225 nonobese subjects. Gln223 Arg polymorphism was genotyped by polymerase chain reaction restriction fragment-length polymorphism. Anthropometric parameters and plasma glucose, total cholesterol, triacylglycerols, high density lipoprotein cholesterol, low density lipoprotein cholesterol, leptin, and insulin were measured.

Results: The Gln223 Arg polymorphism was found to be significantly associated with body mass index, systolic and diastolic blood pressure, and weight only in obese subjects, and with all lipid parameters in both obese and nonobese subjects when Gln/Gln or Gln/Arg genotypes were compared with Arg/Arg genotype.

Conclusions: The LEPR Gln223 is associated with obesity, lipid parameters, and plasma glucose in all subjects, with weight, body mass index, and systolic and diastolic blood pressure only in obese subjects, but showed no association with leptin, insulin, and HOMA-IR in a Pakistani study group.

Keywords: Insulin; Leptin; Leptin receptor; Lipid parameters; Obesity; Single nucleotide polymorphism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Asian People / genetics*
  • Blood Glucose / metabolism
  • Blood Pressure
  • Body Mass Index
  • Cholesterol / blood*
  • Cholesterol, HDL / blood
  • Cholesterol, LDL / blood
  • Female
  • Genotype*
  • Humans
  • Insulin / blood
  • Leptin / blood
  • Lipid Metabolism / genetics
  • Male
  • Middle Aged
  • Obesity / blood
  • Obesity / genetics*
  • Pakistan
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide*
  • Receptors, Leptin / genetics*
  • Triglycerides / blood*


  • Blood Glucose
  • Cholesterol, HDL
  • Cholesterol, LDL
  • Insulin
  • Leptin
  • Receptors, Leptin
  • Triglycerides
  • Cholesterol