Redox Proteomics in Human Biofluids: Sample Preparation, Separation and Immunochemical Tagging for Analysis of Protein Oxidation

Methods Mol Biol. 2016;1303:391-403. doi: 10.1007/978-1-4939-2627-5_23.


Proteomics offers the simultaneous detection of a large number of proteins in a single experiment and can provide important information regarding crucial aspects of specific proteins, particularly post-translational modifications (PTMs). Investigations of oxidative PTMs are currently performed using focused redox proteomics techniques, which rely on gel electrophoresis separations of intact proteins with the final detection of oxidative PTMs being performed by mass spectrometry (MS) analysis. The application of this technique to human biofluids is being subject of increasing investigation and is expected to provide new insights on the oxidative status of the peripheral proteome in neurological diseases such as Alzheimer's disease, towards purposes of early diagnosis and prognosis. This chapter describes all the experimental steps to perform redox proteomics analysis of cerebrospinal fluid and plasma/serum samples.

MeSH terms

  • Analytic Sample Preparation Methods / methods*
  • Blood Proteins / analysis*
  • Blood Proteins / chemistry
  • Blood Proteins / isolation & purification*
  • Blood Proteins / metabolism
  • Cerebrospinal Fluid Proteins / analysis*
  • Cerebrospinal Fluid Proteins / chemistry
  • Cerebrospinal Fluid Proteins / isolation & purification*
  • Cerebrospinal Fluid Proteins / metabolism
  • Collodion / chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Immunochemistry / methods*
  • Isoelectric Focusing
  • Membranes, Artificial
  • Oxidation-Reduction
  • Proteolysis
  • Proteomics / methods*
  • Spectrometry, Mass, Electrospray Ionization
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Trypsin / metabolism


  • Blood Proteins
  • Cerebrospinal Fluid Proteins
  • Membranes, Artificial
  • Collodion
  • Trypsin