Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery

Nat Biotechnol. 2015 Aug;33(8):839-41. doi: 10.1038/nbt.3301. Epub 2015 Aug 3.


Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded in modified mRNA or self-replicating RNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cricetinae
  • Gene Regulatory Networks / genetics*
  • HEK293 Cells
  • Humans
  • RNA / genetics*
  • RNA / metabolism
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism
  • Synthetic Biology / methods*


  • RNA-Binding Proteins
  • RNA