Reduction of butyrate- and methane-producing microorganisms in patients with Irritable Bowel Syndrome

Sci Rep. 2015 Aug 4;5:12693. doi: 10.1038/srep12693.

Abstract

The pathophysiology of irritable bowel syndrome (IBS) remains unclear. Here we investigated the microbiome of a large cohort of patients to identify specific signatures for IBS subtypes. We examined the microbiome of 113 patients with IBS and 66 healthy controls. A subset of these participants provided two samples one month apart. We analyzed a total of 273 fecal samples, generating more than 20 million 16S rRNA sequences. In patients with IBS, a significantly lower microbial diversity was associated with a lower relative abundance of butyrate-producing bacteria (P = 0.002; q < 0.06), in particular in patients with IBS-D and IBS-M. IBS patients who did not receive any treatment harboured a lower abundance of Methanobacteria compared to healthy controls (P = 0.005; q = 0.05). Furthermore, significant correlations were observed between several bacterial taxa and sensation of flatulence and abdominal pain (P < 0.05). Altogether, our findings showed that IBS-M and IBS-D patients are characterized by a reduction of butyrate producing bacteria, known to improve intestinal barrier function, and a reduction of methane producing microorganisms a major mechanism of hydrogen disposal in the human colon, which could explain excess of abdominal gas in IBS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abdominal Pain / microbiology*
  • Abdominal Pain / pathology
  • Abdominal Pain / physiopathology
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Bacteroides / classification
  • Bacteroides / genetics
  • Bacteroides / metabolism
  • Butyrates / metabolism*
  • Case-Control Studies
  • Euryarchaeota / classification
  • Euryarchaeota / genetics
  • Euryarchaeota / metabolism*
  • Feces / microbiology
  • Female
  • Firmicutes / classification
  • Firmicutes / genetics
  • Firmicutes / metabolism
  • Flatulence / microbiology*
  • Flatulence / pathology
  • Flatulence / physiopathology
  • Genetic Variation
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Irritable Bowel Syndrome / microbiology*
  • Irritable Bowel Syndrome / pathology
  • Irritable Bowel Syndrome / physiopathology
  • Male
  • Methane / biosynthesis*
  • Microbiota / genetics
  • Middle Aged
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S / genetics

Substances

  • Butyrates
  • RNA, Ribosomal, 16S
  • Methane