Identification and characterization of proliferative retinopathy-related long noncoding RNAs

Biochem Biophys Res Commun. 2015 Sep 25;465(3):324-30. doi: 10.1016/j.bbrc.2015.07.120. Epub 2015 Aug 1.


Proliferative vitreoretinopathy (PVR) is a serious complication of retinal detachment and vitreoretinal surgery, which can lead to severe vision reduction. Long non-coding RNAs (lncRNAs) play critical roles in many biological processes and disease development. We attempted to determine the role of lncRNAs in the setting of PVR. Microarray analysis revealed that 78 lncRNAs were abnormally expressed in the epiretinal membranes (ERMs) of PVR patients, including 48 up-regulated and 30 down-regulated lncRNA transcripts. We subsequently focus on one lncRNA, MALAT1, and investigated its expression pattern in the biofluid of PVR patients. MALAT1 was significantly up-regulated in the cellular and plasma fraction of peripheral blood in PVR patients. MALAT1 expression was obviously reduced after PVR operation. In vitro experiments revealed the role of MALAT1 in regulating RPE proliferation and migration, which is critical for ERMs formation. This study suggests that lncRNAs are the potential regulators of PVR pathology. MALAT1 is a potential prognostic indicator and a target for the diagnosis and gene therapy for PVR diseases.

Keywords: LncRNA; MALAT1; PVR; Peripheral blood; RPE.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Biomarkers, Tumor / blood
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Genetic Markers / genetics
  • Genetic Predisposition to Disease / genetics*
  • Humans
  • Molecular Sequence Data
  • RNA, Long Noncoding / blood*
  • RNA, Long Noncoding / genetics*
  • Vitreoretinopathy, Proliferative / blood*
  • Vitreoretinopathy, Proliferative / genetics*
  • Vitreoretinopathy, Proliferative / pathology


  • Biomarkers, Tumor
  • Genetic Markers
  • MALAT1 long non-coding RNA, human
  • RNA, Long Noncoding