Optimization of ultrasonic-assisted extraction of antioxidant compounds from Guava (Psidium guajava L.) leaves using response surface methodology

Pharmacogn Mag. 2015 Jul-Sep;11(43):463-9. doi: 10.4103/0973-1296.160451.

Abstract

Objective: To optimization of extraction of antioxidant compounds from guava (Psidium guajava L.) leaves and showed that the guava leaves are the potential source of antioxidant compounds.

Materials and methods: The bioactive polysaccharide compounds of guava leaves (P. guajava L.) were obtained using ultrasonic-assisted extraction. Extraction was carried out according to Box-Behnken central composite design, and independent variables were temperature (20-60°C), time (20-40 min) and power (200-350 W). The extraction process was optimized by using response surface methodology for the highest crude extraction yield of bioactive polysaccharide compounds.

Results: The optimal conditions were identified as 55°C, 30 min, and 240 W. 1,1-diphenyl-2-picryl-hydrazyl and hydroxyl free radical scavenging were conducted.

Conclusion: The results of quantification showed that the guava leaves are the potential source of antioxidant compounds.

Keywords: Antioxidant; guava (Psidium guajava L.) leaves; polysaccharide; ultrasonic-assisted extraction.