Bifidobacterium longum BBMN68-specific modulated dendritic cells alleviate allergic responses to bovine β-lactoglobulin in mice

J Appl Microbiol. 2015 Oct;119(4):1127-37. doi: 10.1111/jam.12923.


Aims: This study was designed to demonstrate the protective effects of Bifidobacterium longum BBMN68-specific modulated dendritic cells (DCs) on allergic inflammation in β-lactoglobulin (BLG)-sensitized mice.

Methods and results: BALB/c mice were sensitized to BLG in accordance with a model of food allergy protocol and given oral BBMN68 daily. BBMN68 was found to significantly reduce BLG-specific hypersensitivity reactions by suppressing the aberrant balance of Th1/Th2 responses with increasing the number of CD4+CD25+Foxp3+ Treg cells in mesenteric lymph nodes (MLN) by 48·1%. The level of CD103+DCs was up-regulated by 136·7 and 56·2% in payer's patches and MLN, respectively, in response to the lower expression levels of cell-surface molecules (CD86 and MHC-II) induced by BBMN68 supplementation. The CD11c+DCs isolated from BBMN68 mice showed 45·6% more Foxp3+ expression in vitro.

Conclusions: These data suggest that BBMN68-specific induction of CD11c+CD103+DCs and semi-mature DCs reduce BLG allergic reactions.

Significance and impact of the study: These data confirm that BBMN68 may be a suitable therapeutic approach to the alleviation of food allergies, and BBMN68-specific induction of CD11c+CD103+DCs and semi-mature DCs are associated with this protection.

Keywords: Bifidobacterium longum BBMN68; T regulatory cell; cow milk allergy; dendritic cell; oral tolerance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bifidobacterium / immunology*
  • Bifidobacterium / isolation & purification
  • Dendritic Cells / drug effects
  • Dendritic Cells / immunology*
  • Female
  • Food Hypersensitivity / drug therapy*
  • Food Hypersensitivity / immunology
  • Humans
  • Lactoglobulins / adverse effects
  • Mice
  • Mice, Inbred BALB C
  • Probiotics / administration & dosage
  • T-Lymphocytes, Regulatory / drug effects
  • T-Lymphocytes, Regulatory / immunology


  • Lactoglobulins