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. 2015 Sep;35(9):4765-75.

Chemoprevention of Azoxymethane-induced Colonic Carcinogenesis in Balb/c Mice Using a Modified Pectin Alginate Probiotic

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  • PMID: 26254367

Chemoprevention of Azoxymethane-induced Colonic Carcinogenesis in Balb/c Mice Using a Modified Pectin Alginate Probiotic

Frederick Odun-Ayo et al. Anticancer Res. .

Abstract

Background: Increased intake of probiotic dietary fibre reduces colonic cancer risk. Modified citrus pectin (MCP) requires optimal bioactivity to inhibit galectin-3 (GAL-3) and vascular endothelial growth factor (VEGF). This study evaluated the preventative effect of modified pectin alginate (MCPA) probiotic microbeads on azoxymethane (AOM)-induced colonic carcinogenesis in Balb/c mice.

Materials and methods: Optimization of AOM dose duration: 10-15 mg/kg was administered for 2-4 weeks. The optimal AOM dose was initiated prior to intake of MCPA, alginate probiotic (AP) microbeads and MCP in Balb/c mice for 16 weeks; samples were analyzed for colonic histopathology and immunohistochemistry.

Results: AOM at 15 mg/kg for 4 weeks induced optimal GAL-3 and VEGF immunostaining. Furthermore, MCPA treatment reduced GAL-3 expression in the colon of AOM-treated mice compared to MCP.

Conclusion: MCPA probiotic microbeads increase bioactivity and chemopreventative effect against pre-cancerous colonic lesions and adenocarcinoma through inhibition of GAL-3 and VEGF in the Balb/c mouse model of colonic carcinogenesis.

Keywords: Azoxymethane; colonic cancer; galectin-3; immunohistochemistry; modified citrus pectin; probiotic.

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