Chemoprevention of Azoxymethane-induced Colonic Carcinogenesis in Balb/c mice Using a Modified Pectin Alginate Probiotic

Anticancer Res. 2015 Sep;35(9):4765-75.


Background: Increased intake of probiotic dietary fibre reduces colonic cancer risk. Modified citrus pectin (MCP) requires optimal bioactivity to inhibit galectin-3 (GAL-3) and vascular endothelial growth factor (VEGF). This study evaluated the preventative effect of modified pectin alginate (MCPA) probiotic microbeads on azoxymethane (AOM)-induced colonic carcinogenesis in Balb/c mice.

Materials and methods: Optimization of AOM dose duration: 10-15 mg/kg was administered for 2-4 weeks. The optimal AOM dose was initiated prior to intake of MCPA, alginate probiotic (AP) microbeads and MCP in Balb/c mice for 16 weeks; samples were analyzed for colonic histopathology and immunohistochemistry.

Results: AOM at 15 mg/kg for 4 weeks induced optimal GAL-3 and VEGF immunostaining. Furthermore, MCPA treatment reduced GAL-3 expression in the colon of AOM-treated mice compared to MCP.

Conclusion: MCPA probiotic microbeads increase bioactivity and chemopreventative effect against pre-cancerous colonic lesions and adenocarcinoma through inhibition of GAL-3 and VEGF in the Balb/c mouse model of colonic carcinogenesis.

Keywords: Azoxymethane; colonic cancer; galectin-3; immunohistochemistry; modified citrus pectin; probiotic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / pharmacology
  • Alginates / therapeutic use*
  • Animals
  • Azoxymethane
  • Carcinogenesis / drug effects
  • Carcinogenesis / metabolism
  • Carcinogenesis / pathology*
  • Chemoprevention*
  • Colon / drug effects
  • Colon / metabolism
  • Colon / pathology
  • Colonic Neoplasms / drug therapy*
  • Colonic Neoplasms / pathology*
  • Disease Models, Animal
  • Galectins / metabolism
  • Glucuronic Acid / pharmacology
  • Glucuronic Acid / therapeutic use
  • Hexuronic Acids / pharmacology
  • Hexuronic Acids / therapeutic use
  • Immunohistochemistry
  • Male
  • Mice, Inbred BALB C
  • Pectins
  • Probiotics / pharmacology
  • Probiotics / therapeutic use*
  • Vascular Endothelial Growth Factor A / metabolism


  • Alginates
  • Galectins
  • Hexuronic Acids
  • Vascular Endothelial Growth Factor A
  • Pectins
  • Glucuronic Acid
  • Azoxymethane