Elutriation for Cell Cycle Synchronization in Fission Yeast

Methods Mol Biol. 2016:1342:149-55. doi: 10.1007/978-1-4939-2957-3_7.

Abstract

Cell synchronization is a powerful technique for studying the eukaryotic cell cycle events precisely. The fission yeast is a rod-shaped cell whose growth is coordinated with the cell cycle. Monitoring the cellular growth of fission yeast is a relatively simple way to measure the cell cycle stage of a cell. Here, we describe a detailed method of unperturbed cell synchronization, named centrifugal elutriation, for fission yeast.

MeSH terms

  • Cell Count
  • Cell Cycle*
  • Cell Nucleus / metabolism
  • Centrifugation / instrumentation
  • Centrifugation / methods*
  • Schizosaccharomyces / cytology*
  • Sterilization