Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence

Methods Mol Biol. 2016;1342:173-83. doi: 10.1007/978-1-4939-2957-3_9.

Abstract

Immunofluorescence can be a powerful tool to detect protein levels, intracellular localization, and post-translational modifications. However, standard immunofluorescence provides only a still picture and thus lacks temporal information. Here, we describe a method to extract temporal information from immunofluorescence images of fixed cells. In addition, we provide an optional protocol that uses micropatterns, which increases the accuracy of the method. These methods allow assessing how protein levels, intracellular localization, and post-translational modifications change through the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle*
  • Fluorescent Antibody Technique / methods*
  • Image Processing, Computer-Assisted
  • Kinetics
  • Protein Processing, Post-Translational*