Isolation of segmented filamentous bacteria from complex gut microbiota

Biotechniques. 2015 Aug 1;59(2):94-8. doi: 10.2144/000114319. eCollection 2015 Aug.


Segmented filamentous bacteria (SFB) modulate the ontogeny of the immune system, and their presence can significantly affect mouse models of disease. Until recently, the inability to successfully culture SFB has made controlled studies on the mechanisms by which these bacteria exert their influence problematic. Here, we report a new method for selecting SFB from complex microbial mixtures, providing researchers a simple and cost-effective means to prepare pure infective inocula for prospective studies and also to compare individual SFB isolates.

Keywords: Candidatus Arthromitus; SFB; commensal; culture; microbiota; segmented filamentous bacteria.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteriological Techniques*
  • Disease Models, Animal
  • Gastrointestinal Microbiome*
  • Gram-Negative Bacteria / isolation & purification*
  • Mice