Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport

J Am Chem Soc. 2015 Aug 26;137(33):10464-7. doi: 10.1021/jacs.5b05672. Epub 2015 Aug 11.


Artificial ligands of streptavidin (ALiS) with association constants of ∼10(6) M(-1) were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS system provides a novel, practical compound-dependent methodology for repeated reversible cycling of protein localization between intracellular organella.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Drug Evaluation, Preclinical
  • Intracellular Space / metabolism*
  • Kinetics
  • Ligands
  • Models, Molecular
  • Permeability
  • Protein Conformation
  • Protein Transport
  • Streptavidin / chemistry
  • Streptavidin / metabolism*


  • Ligands
  • Streptavidin