[Candidatus "Jettenia Moscovienalis" Sp. Nov., a New Species of Bacteria Carrying Out Anaerobic Ammonium Oxidation]

Mikrobiologiia. Mar-Apr 2015;84(2):236-43.
[Article in Russian]

Abstract

A new species of bacteria oxidizing ammonium with nitrite under anoxic conditions was isolated from the activated sludge of a semi-industrial bioreactor treating digested sludge of the Kuryanovo wastewater treatment plant (Moscow, Russia). Physiological, morphological, and molecular genetic characterization of the isolate was carried out. The cells were ovoid (-0.5 x 0.8 μm), with the intracellular membrane structures characteristic of anammox bacteria (anammoxosome and paryphoplasm); unlike other anammox bacteria, it possessed extensive intracellular membrane structures located in layers parallel to the cytoplasmic membrane, but never close to the anammoxosome. The cells formed aggregates 5-28 μm in diameter and readily attached to solid surfaces. The cells were morphologically labile, easily plasmolyzed, and lost their content. Doubling time was 28 days, μ(max) = 0.025 day(-1); optimal temperature and pH for growth were 20-45 degrees C and 8.0, respectively. Phylogenetic analysis of the 16S rRNA gene sequences suggested its classification as a new species of the candidate genus Jettenia (order Planctomycetales). The name Candidatus "Jettenia moscovienalis" sp. nov. was proposed for the new bacterium.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ammonia / metabolism*
  • Anaerobiosis
  • Genes, rRNA*
  • Hydrogen-Ion Concentration
  • Intracellular Membranes / ultrastructure
  • Nitrites / metabolism
  • Oxidation-Reduction
  • Phylogeny
  • Planctomycetales / classification*
  • Planctomycetales / isolation & purification
  • Planctomycetales / metabolism*
  • Planctomycetales / ultrastructure
  • RNA, Ribosomal, 16S / genetics
  • Waste Water / microbiology
  • Water Microbiology*

Substances

  • Nitrites
  • RNA, Ribosomal, 16S
  • Waste Water
  • Ammonia