Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Aug 12;15:159.
doi: 10.1186/s12866-015-0499-0.

Effect of the Synthetic Cannabinoid HU-210 on Quorum Sensing and on the Production of Quorum Sensing-Mediated Virulence Factors by Vibrio Harveyi

Affiliations
Free PMC article

Effect of the Synthetic Cannabinoid HU-210 on Quorum Sensing and on the Production of Quorum Sensing-Mediated Virulence Factors by Vibrio Harveyi

Divya Soni et al. BMC Microbiol. .
Free PMC article

Abstract

Background: Bacterial populations communicate through the cell density-dependent mechanism of quorum sensing (QS). Vibrio harveyi, one of the best studied model organisms for QS, was used to explore effects of the synthetic cannabinoid HU-210 on QS and different QS-regulated physiological processes in bacteria.

Results: Analysis of QS-regulated bioluminescence in wild-type and mutant strains of V. harveyi revealed that HU-210 affects the autoinducer-2 (AI-2) pathway, one of three known QS cascades of V. harveyi. Furthermore, QS-mediated biofilm formation and swimming motility in the mutant strain BB152 (AI-1(-), AI-2(+)) were significantly reduced in the presence of HU-210. HU-210 inhibited QS-mediated virulence factor production without any inhibitory effect on bacterial growth. It also alters the expression of several genes, which are regulated by QS, specifically downregulating the genes of the AI-2 QS cascade.

Conclusion: First evidence is being provided for interference of bacterial signal-transduction systems by a synthetic cannabinoid. The effect of HU-210 was specific to the AI-2 cascade in V. harveyi. AI-2 is known as a "universal autoinducer" and interference with its activity opens a broad spectrum of applications for synthetic cannabinoids in future research as a potential anti-QS agent.

Figures

Fig. 1
Fig. 1
Chemical structure of the synthetic cannabinoid HU-210, used in this study
Fig. 2
Fig. 2
Effect of HU-210 on growth and bioluminescence production of V. harveyi. (a) Comparison of relative bioluminescence production by V. harveyi BB120 (wild type), MM30 (AI-1+, AI-2) and BB152 (AI-1, AI-2+) with different HU-210 concentrations, presented as area under the curve. (b) Growth curve of V. harveyi BB152 with different HU-210 concentrations. (c) Relative bioluminescence production by V. harveyi BB170 (Sensor-1, Sensor-2+) with different HU-210 concentrations. (d) Relative bioluminescence production by V. harveyi BB152 with different HU-210 concentrations and simultaneous supplementation with exogenous AI-1 isolated from V. harveyi MM30 (AI-1+, AI-2), presented as area under the curve. Presented data are means and SD of three independent experiments, each performed in triplicate.*P < 0.05 compared with control
Fig. 3
Fig. 3
DNA quantification by qPCR. Comparison of quantified DNA of V. harveyi BB120 (wild type), MM30 (AI-1+, AI-2) and BB152 (AI-1, AI-2+) biofilm formed with or without different concentrations of HU-210. Presented data are means and SD of three independent experiments, each performed in triplicate.*P < 0.05 compared with control
Fig. 4
Fig. 4
Analysis of V. harveyi biofilms by CLSM. (a) Comparison of biofilm depth of V. harveyi BB120 (wild type), MM30 (AI-1+, AI-2) and BB152 (AI-1, AI-2+) formed with or without different concentrations of HU-210. Presented data are means and SD of three independent experiments.*P < 0.05 compared with control. (b) V. harveyi BB120 (wild type), MM30 (AI-1+, AI-2) and BB152 (AI-1, AI-2+) biofilms grown with or without HU-210 (200 μg/ml) and stained with SYTO 9 dye (green), which stains live bacteria, and with concanavalin A–Alexa Fluor 647 conjugates (blue), which stain extracellular polysaccharides. Biofilm depth of V. harveyi BB120 (wild type), MM30 (AI-1+, AI-2) and BB152 (AI-1, AI-2+) biofilms in control (without HU-210) is 85 μm, 80 μm ,95 μm and in samples with HU-210 (200 μg/ml) is 80 μm, 85 μm, 55 μm respectively
Fig. 5
Fig. 5
Comparison of swimming motility of V. harveyi BB120 (wild type), MM30 (AI-1+, AI-2) and BB152 (AI-1, AI-2+) with different concentrations of HU-210 quantified using Image J Software. Presented data are means and SD of two independent experiments (*P < 0.05)

Similar articles

See all similar articles

Cited by 5 articles

References

    1. Fuqua WC, Winans SC, Greenberg EP. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. J Bacteriol. 1994;176(2):269–275. - PMC - PubMed
    1. Miller MB, Bassler BL. Quorum sensing in bacteria. Annu Rev Microbiol. 2001;55:165–199. doi: 10.1146/annurev.micro.55.1.165. - DOI - PubMed
    1. Hammer BK, Bassler BL. Quorum sensing controls biofilm formation in Vibrio cholerae. Mol Microbiol. 2003;50(1):101–104. doi: 10.1046/j.1365-2958.2003.03688.x. - DOI - PubMed
    1. Henke JM, Bassler BL. Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyi. J Bacteriol. 2004;186(20):6902–6914. doi: 10.1128/JB.186.20.6902-6914.2004. - DOI - PMC - PubMed
    1. Waters CM, Bassler BL. The Vibrio harveyi quorum-sensing system uses shared regulatory components to discriminate between multiple autoinducers. Genes Dev. 2006;20(19):2754–2767. doi: 10.1101/gad.1466506. - DOI - PMC - PubMed

MeSH terms

Feedback