Clinical, Radiographic, and Histologic Analysis of the Effects of Acemannan Used in Direct Pulp Capping of Human Primary Teeth: Short-Term Outcomes

Odontology. 2016 Sep;104(3):329-37. doi: 10.1007/s10266-015-0215-4. Epub 2015 Aug 12.


Acemannan has been previously reported as a direct pulp-capping agent in animal study. This natural material demonstrated its biocompatibility and enhanced reparative dentin formation. The objective of this study was to investigate the action of acemannan as a direct pulp-capping material in human primary teeth with deep caries. Forty-two deeply carious mandibular primary molars from 37 children, aged 7-11 years old diagnosed with reversible pulpitis were studied. After completely removing the infected dentine, teeth with a pinpoint pulpal exposure were randomly divided into two treatment groups: acemannan or calcium hydroxide. A glass-ionomer cement base was applied to all teeth prior to restoration with stainless steel crowns. Clinical and radiographic evaluation was performed 6 months post-treatment. The teeth due to exfoliate were extracted and histopathologically evaluated for inflammation, dentine bridge formation, and soft tissue organization. At 6 months, the overall clinical and radiographic success rates of direct pulp capping with acemannan and calcium hydroxide at 6 months were 72.73 and 70.0 %, respectively. The histopathological results indicated that the acemannan-treated group had significantly better histopathological responses compared with the calcium hydroxide-treated group (p < 0.05). These data suggest acemannan offers a valuable alternative biomaterial for vital pulp therapy in primary teeth.

Keywords: Acemannan; Aloe vera; Biomaterial; Clinical study; Histopathological evaluation; Vital pulp therapy.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Calcium Hydroxide / therapeutic use
  • Child
  • Dental Pulp / drug effects
  • Dental Pulp Capping / methods*
  • Female
  • Humans
  • Male
  • Mannans / therapeutic use*
  • Molar
  • Plant Extracts / therapeutic use*
  • Pulpitis / drug therapy*
  • Tooth, Deciduous
  • Treatment Outcome


  • Mannans
  • Plant Extracts
  • Calcium Hydroxide
  • acemannan