Inhibitory Effects of Lactobacillus Fermentum on Microbial Growth and Biofilm Formation

Arch Microbiol. 2015 Oct;197(8):1027-32. doi: 10.1007/s00203-015-1140-1. Epub 2015 Aug 13.

Abstract

Beneficial effects of Lactobacilli have been reported, and lactic bacteria are employed for conservation of foods. Therefore, the effects of a Lactobacillus fermentum strain were analyzed regarding inhibitory effects on staphylococci, Candida albicans and enterotoxigenic enterobacteria by transmission electron microscopy (TEM). TEM of bacterial biofilms was performed using cocultures of bacteriocin-producing L. fermentum 97 with different enterotoxigenic strains: Staphylococcus epidermidis expressing the ica gene responsible for biofilm formation, Staphylococcus aureus producing enterotoxin type A, Citrobacter freundii, Enterobacter cloaceae, Klebsiella oxytoca, Proteus mirabilis producing thermolabile and thermostable enterotoxins determined by elt or est genes, and Candida albicans. L. fermentum 97 changed morphological features and suppressed biofilm formation of staphylococci, enterotoxigenic enterobacteria and Candida albicans; a marked transition to resting states, a degradation of the cell walls and cytoplasm, and a disruption of mature bacterial biofilms were observed, the latter indicating efficiency even in the phase of higher cell density.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiosis*
  • Bacteria / growth & development
  • Bacterial Physiological Phenomena*
  • Bacteriocins / metabolism
  • Biofilms*
  • Candida albicans / growth & development
  • Candida albicans / physiology*
  • Cell Wall / metabolism
  • Citrobacter freundii / growth & development
  • Citrobacter freundii / physiology
  • Enterobacteriaceae / growth & development
  • Enterobacteriaceae / physiology
  • Lactobacillus fermentum / physiology*
  • Microscopy, Electron, Transmission
  • Proteus mirabilis / growth & development
  • Proteus mirabilis / physiology
  • Staphylococcus aureus / growth & development
  • Staphylococcus aureus / physiology*

Substances

  • Bacteriocins