The H2S-producing enzyme CSE is dispensable for the processing of inflammatory and neuropathic pain

Brain Res. 2015 Oct 22;1624:380-389. doi: 10.1016/j.brainres.2015.07.058. Epub 2015 Aug 10.


Accumulating lines of evidence indicate that hydrogen sulfide (H2S) contributes to the processing of chronic pain. However, the sources of H2S production in the nociceptive system are poorly understood. Here we investigated the expression of the H2S releasing enzyme cystathionine γ-lyase (CSE) in the nociceptive system and characterized its role in chronic pain signaling using CSE deficient mice. We show that paw inflammation and peripheral nerve injury led to upregulation of CSE expression in dorsal root ganglia. However, conditional knockout mice lacking CSE in sensory neurons as well as global CSE knockout mice demonstrated normal pain behaviors in inflammatory and neuropathic pain models as compared to WT littermates. Thus, our results suggest that CSE is not critically involved in chronic pain signaling in mice and that sources different from CSE mediate the pain relevant effects of H2S.

Keywords: CSE; CTH; Chronic pain; Dorsal root ganglia; Hydrogen sulfide; Knockout mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cystathionine gamma-Lyase / genetics
  • Cystathionine gamma-Lyase / metabolism*
  • Disease Models, Animal
  • Formaldehyde / toxicity
  • Ganglia, Spinal / metabolism*
  • Gene Expression Regulation / genetics
  • Hydrogen Sulfide / metabolism*
  • Hyperalgesia / etiology
  • Hyperalgesia / metabolism
  • Inflammation / chemically induced
  • Inflammation / metabolism*
  • Inflammation / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Motor Activity / physiology
  • Neuralgia / metabolism*
  • Neuralgia / pathology
  • Pain Measurement
  • Spinal Cord / metabolism
  • Up-Regulation
  • Zymosan / pharmacology


  • Formaldehyde
  • Zymosan
  • Cystathionine gamma-Lyase
  • Hydrogen Sulfide