Hsa-miR-1 suppresses breast cancer development by down-regulating K-ras and long non-coding RNA MALAT1

Int J Biol Macromol. 2015 Nov;81:491-7. doi: 10.1016/j.ijbiomac.2015.08.016. Epub 2015 Aug 11.

Abstract

MicroRNAs exert their functions by mainly regulating coding genes or long non-coding RNA expression. In the present study, we reported that hsa-miR-1 was down-regulated in breast cancer tissues. Restoration of miR-1 in breast cancer cells inhibited proliferation, motility and increased apoptosis in vitro. MiR-1 functioned as a tumor suppressor by targeting K-RAS and MALAT1. In addition, the effects of up-regulation of miR-1 were similar to that of silencing K-RAS and MALAT1 in breast cancer cells. In vivo study indicated that restoration of miR-1 inhibited tumor growth and metastasis. Patients with low miR-1 expression had poorer overall survival time than those with high miR-1 expression. Our findings emphasized the potential role of miR-1 as tumor suppressive miRNA in breast cancer.

Keywords: K-RAS; MALAT1; miR-1.

MeSH terms

  • 3' Untranslated Regions
  • Adult
  • Aged
  • Animals
  • Apoptosis / genetics
  • Base Sequence
  • Binding Sites
  • Biomarkers, Tumor
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Cell Transformation, Neoplastic / genetics*
  • Disease Models, Animal
  • Down-Regulation
  • Ectopic Gene Expression
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • MicroRNAs / chemistry
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasm Metastasis
  • Neoplasm Staging
  • Proto-Oncogene Proteins p21(ras) / chemistry
  • Proto-Oncogene Proteins p21(ras) / genetics*
  • RNA Interference*
  • RNA, Long Noncoding / chemistry
  • RNA, Long Noncoding / genetics*
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • Tumor Burden / genetics
  • Xenograft Model Antitumor Assays

Substances

  • 3' Untranslated Regions
  • Biomarkers, Tumor
  • KRAS protein, human
  • MALAT1 long non-coding RNA, human
  • MIRN1 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • RNA, Messenger
  • Proto-Oncogene Proteins p21(ras)