DNA-stabilized bimetallic nanozyme and its application on colorimetric assay of biothiols

Biosens Bioelectron. 2015 Dec 15:74:1038-46. doi: 10.1016/j.bios.2015.08.001. Epub 2015 Aug 4.

Abstract

This paper reports a facile synthesis of bimetallic AuxPty nanozyme using C-rich oligonucleotide as the nucleation template, with high peroxidase-like activity and thiophilicity associated with the ratio of x:y. The efficient Au2Pt1 nanozyme possesses highly peroxidase mimicking activity with the Km value of 0.088 mM toward 3,3',5,5'-tetramethylbenzidine and 196 mM toward hydrogen peroxide. It is the first report to explore colorimetric assays for biothiols with high sensitivity and selectivity against non-thiol-containing amino acids, on the basis of thiol-induced inhibition of peroxidase-like activities of metal nanoparticles. By using Au2Pt1 nanozyme, the limit of detection (LOD) is 3.5 nM for cysteine and 1.6 nM for homocysteine. This DNA-stabilized bimetallic AuxPty nanozyme shows a promising application in quantitative detection of biothiols in biological fluids.

Keywords: DNA; Gold; Peroxidase; Platinum; Thiophilicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alloys / chemistry
  • Biosensing Techniques / instrumentation*
  • Colorimetry / instrumentation*
  • DNA / chemistry*
  • Equipment Design
  • Equipment Failure Analysis
  • Excipients / chemistry
  • Gold / chemistry
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / ultrastructure
  • Peroxidase / chemistry*
  • Sulfhydryl Compounds / analysis*

Substances

  • Alloys
  • Excipients
  • Sulfhydryl Compounds
  • Gold
  • DNA
  • Peroxidase