This article includes the distribution records and updated checklist of mosquitoes (Culicidae, Diptera) from the Lao People's Democratic Republic (PDR), based on the literature, specimens deposited at the US National Museum of Natural History mosquito collections, and our recent field collections from the Nakai District, Khammuane Province. Ten of 101 species in the updated checklist of mosquitoes are new records for the Lao PDR.