[Effects of Panax notoginseng saponins on proliferation, apoptosis and cell cycle of K562 cells in vitro and the mechanisms]

Nan Fang Yi Ke Da Xue Xue Bao. 2015 Aug;35(8):1103-9.
[Article in Chinese]

Abstract

Objective: To investigate the effects of Panax notoginseng saponins (PNS) on the proliferation, apoptosis and cell cycle of K562 cells and explore the molecular mechanisms underlying these effects.

Methods: PNS-induced growth inhibition of K562 cells was detected by MTT assay; the cell apoptosis was evaluated by AO/EB staining and Annexin V-FITC/ PI staining; flow cytometry was used to detect cell cycle changes in the treated cells. The mRNA expressions of the molecules in mTOR signaling pathway were examined by RT-PCR, and the cellular expressions of cleaved caspeas-3, cyclin D1 and major proteins in mTOR signaling pathway were detected using Western blotting.

Results: MTT assay showed that treatment with 100-800 µg/mL PNS significantly inhibited the proliferation, promoted the cell apoptosis, and caused cell cycle arrest in G0/G1 phase in K562 cells. Western blotting revealed increased protein expression of cleaved caspase-3 and decreased expression of cyclin D1 in PNS-treated cells, in which the proteins expressions of mTOR, p-mTOR, p-p70S6K and p-4E-BP 1 and the mRNA expression of mTOR were all decreased.

Conclusion: PNS can inhibit the proliferation, induce apoptosis and cause cell cycle arrest in K562 cells possibly by up-regulating cleaved caspase 3 and down-regulating cyclin D1 and mTOR signaling pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects*
  • Caspase 3 / metabolism
  • Cell Cycle / drug effects*
  • Cell Cycle Checkpoints
  • Cell Proliferation / drug effects*
  • Cyclin D1 / metabolism
  • Humans
  • K562 Cells / drug effects
  • Panax notoginseng / chemistry*
  • Saponins / chemistry*
  • Signal Transduction
  • TOR Serine-Threonine Kinases / metabolism
  • Up-Regulation

Substances

  • CCND1 protein, human
  • Saponins
  • Cyclin D1
  • MTOR protein, human
  • TOR Serine-Threonine Kinases
  • CASP3 protein, human
  • Caspase 3