Comparison of Abtectcell III and Diamed red cell antibody screening kit for detection of clinically significant red cells alloantibody

Malays J Pathol. 2015 Aug;37(2):109-14.

Abstract

Antibody screening is important for the antenatal screening and pre-transfusion tests. This study aimed to compare the MUT/Mur kodecytesAbtectcell III (CSL Abtectcell III) red cell antibody screening kit with DiaMed ID-Dia Cell I-II-III Asia that was then used in our laboratory. In this study, 125 samples were randomly chosen, with 67 samples of known antibody specificities and 58 samples identified as negative for antibody, as the negative control. Concordant negative results were obtained in 57 out of 58 antibody negative samples. Concordant antibody positive results with both reagents were seen in 49 out of 67 samples. There were 18 discrepant results of antibody screening with CSL Abtetcell III (16/18 for vMNS antibodies). The sensitivity and specificity for CSL Abtectcell III were 73.0% and 98.3% respectively. In conclusion, the CSL Abtectcell III reagent would be an acceptable alternative for screening of red cell alloantibodies. It was able to detect all the clinically significant alloantibodies.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asian Continental Ancestry Group
  • Blood Group Antigens / immunology*
  • Cross-Sectional Studies
  • Humans
  • Immunologic Techniques*
  • Isoantibodies / blood*
  • Malaysia
  • Sensitivity and Specificity

Substances

  • Blood Group Antigens
  • Isoantibodies