Multimodal Chemosensory Circuits Controlling Male Courtship in Drosophila

Neuron. 2015 Sep 2;87(5):1036-49. doi: 10.1016/j.neuron.2015.07.025. Epub 2015 Aug 13.


Throughout the animal kingdom, internal states generate long-lasting and self-perpetuating chains of behavior. In Drosophila, males instinctively pursue females with a lengthy and elaborate courtship ritual triggered by activation of sexually dimorphic P1 interneurons. Gustatory pheromones are thought to activate P1 neurons but the circuit mechanisms that dictate their sensory responses to gate entry into courtship remain unknown. Here, we use circuit mapping and in vivo functional imaging techniques to trace gustatory and olfactory pheromone circuits to their point of convergence onto P1 neurons and reveal how their combined input underlies selective tuning to appropriate sexual partners. We identify inhibition, even in response to courtship-promoting pheromones, as a key circuit element that tunes and tempers P1 neuron activity. Our results suggest a circuit mechanism in which balanced excitation and inhibition underlie discrimination of prospective mates and stringently regulate the transition to courtship in Drosophila.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Acetylcholine / pharmacology
  • Analysis of Variance
  • Animals
  • Animals, Genetically Modified
  • Arthropod Antennae / cytology
  • Central Nervous System / cytology*
  • Chemoreceptor Cells / drug effects
  • Chemoreceptor Cells / physiology*
  • Courtship*
  • Drosophila / physiology*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism
  • Female
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Male
  • Nerve Net / drug effects
  • Nerve Net / physiology*
  • Optogenetics
  • Pheromones / pharmacology
  • Pheromones / physiology*
  • Taste / physiology*
  • Transcription Factors / metabolism
  • Xanthenes / metabolism


  • Drosophila Proteins
  • GAL4 protein, Drosophila
  • Pheromones
  • Transcription Factors
  • Xanthenes
  • Green Fluorescent Proteins
  • Texas red
  • Acetylcholine