Histology and immunohistology of bone marrow biopsy in multiple myeloma

Eur J Haematol Suppl. 1989;51:52-9. doi: 10.1111/j.1600-0609.1989.tb01493.x.

Abstract

B5-fixed/paraffin-embedded Jamshidi needle biopsies from 125 multiple myeloma patients were reviewed according to both morphological and immunohistological criteria. At microscopic examination, the following parameters were evaluated: i) grade of malignancy (low = 56; intermediate = 50; high = 19); ii) growth pattern (interstitial +/- sheets/nodules = 90; nodular = 13; packed marrow = 18; sarcomatous = 4); III) histological stage (I = 64; II = 35; III = 26). Comparison of the findings in trephine biopsies and aspirates showed that in 30% of the cases the latter led to an underestimation of the tumor burden. Immunohistochemical determination of Ig easily allowed: i) differential diagnosis from exuberant reactive plasmacytosis; ii) recognition and counting of neoplastic plasma cells; iii) detection of minimal residual disease after treatment. Immunohistochemistry also confirmed phenotypic aberration of neoplastic plasma cells, showing positivity for CD45, EMA, and cytokeratins in 14%, 59%, and 25% of the cases, respectively. Furthermore, it displayed expression of the P-glycoprotein in 4/8 resistant cases. These findings underline that routinely processed Jamshidi needle biopsies can be of great value in the study of patients with multiple myeloma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Antigens, Neoplasm / analysis
  • Biopsy, Needle*
  • Bone Marrow / immunology
  • Bone Marrow / pathology*
  • Bone Marrow Examination / methods*
  • Hodgkin Disease / diagnosis
  • Humans
  • Immunohistochemistry
  • Leukemia / diagnosis
  • Multiple Myeloma / diagnosis
  • Multiple Myeloma / immunology
  • Multiple Myeloma / pathology*
  • Neoplasm Proteins / analysis
  • Neoplasms, Multiple Primary / diagnosis

Substances

  • Antigens, Neoplasm
  • Neoplasm Proteins