ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors

Nat Methods. 2015 Oct;12(10):963-965. doi: 10.1038/nmeth.3542. Epub 2015 Aug 17.


Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatin Immunoprecipitation / economics
  • Chromatin Immunoprecipitation / instrumentation
  • Chromatin Immunoprecipitation / methods*
  • Genome, Human
  • Histones / metabolism*
  • Humans
  • K562 Cells
  • Transcription Factors / analysis
  • Transcription Factors / metabolism*


  • Histones
  • Transcription Factors