Quantification of viral genome in cord blood donors by real time PCR to investigate human herpesvirus type 8 active infection

Neda Golchin; Maryam Kheirandish; Zohreh Sharifi; Shahram Samiee; Parviz Kokhaei; Zahra Pourpak

doi:10.1016/j.transci.2015.08.001

Quantification of viral genome in cord blood donors by real time PCR to investigate human herpesvirus type 8 active infection

Transfus Apher Sci. 2015 Dec;53(3):378-80. doi: 10.1016/j.transci.2015.08.001. Epub 2015 Aug 7.

Authors

Neda Golchin¹, Maryam Kheirandish², Zohreh Sharifi³, Shahram Samiee³, Parviz Kokhaei⁴, Zahra Pourpak⁵

Affiliations

¹ Department of Immunology, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
² Department of Immunology, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Electronic address: m.kheirandish@ibto.ir.
³ Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
⁴ Cancer Research Center and Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran.
⁵ Department of Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran.

PMID: 26283174
DOI: 10.1016/j.transci.2015.08.001

Abstract

Umbilical cord blood (UCB) is one of the most important sources of hematopoietic stem cells which can be used for transplantation. The transplanted CB stem cells might cause infections in recipients. The aim of this study is to evaluate Human Herpes Virus8 (HHV8) as a Rhadinovirus among CB samples in order to assess safety of cord blood stem cells transplantation. To assess this aim, we surveyed 800 cord blood specimens by Real Time PCR.The overall HHV8 incidence in cord blood mononuclear cells was 1.38% and none of them was in lytic phase of HHV8. The authors suggest further HHV8 study on CB samples for transplantation.

Keywords: Cord blood; HHV8; Real time PCR.

MeSH terms

Blood Donors*
Cord Blood Stem Cell Transplantation*
Female
Fetal Blood / virology*
Genome, Viral*
Herpesvirus 8, Human*
Humans
Leukocytes, Mononuclear / virology*
Male
Real-Time Polymerase Chain Reaction / methods*