Regulation of the Contribution of Integrin to Cell Attachment on Poly(2-Methoxyethyl Acrylate) (PMEA) Analogous Polymers for Attachment-Based Cell Enrichment

PLoS One. 2015 Aug 19;10(8):e0136066. doi: 10.1371/journal.pone.0136066. eCollection 2015.

Abstract

Cell enrichment is currently in high demand in medical engineering. We have reported that non-blood cells can attach to a blood-compatible poly(2-methoxyethyl acrylate) (PMEA) substrate through integrin-dependent and integrin-independent mechanisms because the PMEA substrate suppresses protein adsorption. Therefore, we assumed that PMEA analogous polymers can change the contribution of integrin to cell attachment through the regulation of protein adsorption. In the present study, we investigated protein adsorption, cell attachment profiles, and attachment mechanisms on PMEA analogous polymer substrates. Additionally, we demonstrated the possibility of attachment-based cell enrichment on PMEA analogous polymer substrates. HT-1080 and MDA-MB-231 cells started to attach to poly(butyl acrylate) (PBA) and poly(tetrahydrofurfuryl acrylate) (PTHFA), on which proteins could adsorb well, within 1 h. HepG2 cells started to attach after 1 h. HT-1080, MDA-MB-231, and HepG2 cells started to attach within 30 min to PMEA, poly(2-(2-methoxyethoxy) ethyl acrylate-co-butyl acrylate) (30:70 mol%, PMe2A) and poly(2-(2-methoxyethoxy) ethoxy ethyl acrylate-co-butyl acrylate) (30:70 mol%, PMe3A), which suppress protein adsorption. Moreover, the ratio of attached cells from a cell mixture can be changed on PMEA analogous polymers. These findings suggested that PMEA analogous polymers can be used for attachment-based cell enrichment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylates / chemistry*
  • Biocompatible Materials / chemistry*
  • Cell Adhesion / physiology*
  • Cell Culture Techniques
  • Cell Line, Tumor
  • Focal Adhesions / metabolism*
  • Hep G2 Cells
  • Humans
  • Integrins / metabolism
  • Polymers / chemistry*
  • Surface Properties

Substances

  • Acrylates
  • Biocompatible Materials
  • Integrins
  • Polymers
  • poly(2-methoxyethylacrylate)

Grant support

This work was supported by the Funding Program for the Next Generation World-Leading Researchers (NEXT Program) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan and a Grant-in-Aid for Young Scientists (A) (26702016) from MEXT, Japan.