Attenuation of post-infarction remodeling in rats by sustained myocardial growth hormone administration

Growth Factors. 2015;33(4):250-8. doi: 10.3109/08977194.2015.1072527. Epub 2015 Aug 12.


Prevention of left ventricular remodeling is an important therapeutic target post-myocardial infarction. Experimentally, treatment with growth hormone (GH) is beneficial, but sustained local administration has not been thoroughly investigated. We studied 58 rats (322 ± 4 g). GH was administered via a biomaterial-scaffold, following in vitro and in vivo evaluation of degradation and drug-release curves. Treatment consisted of intra-myocardial injection of saline or alginate-hydrogel, with or without GH, 10 min after permanent coronary artery ligation. Echocardiographic and histologic remodeling-indices were examined 3 weeks post-ligation, followed by immunohistochemical evaluation of angiogenesis, collagen, macrophages and myofibroblasts. GH-release completed at 3 days and alginate-degradation at ∼7 days. Alginate + GH consistently improved left ventricular end-diastolic and end-systolic diameters, ventricular sphericity, wall tension index and infarct-thickness. Microvascular-density and myofibroblast-count in the infarct and peri-infarct areas were higher after alginate + GH. Macrophage-count and collagen-content did not differ between groups. Early, sustained GH-administration enhances angiogenesis and myofibroblast-activation and ameliorates post-infarction remodeling.

Keywords: Alginate; angiogenesis; growth hormone; myocardial infarction; myofibroblasts; ventricular remodeling.

MeSH terms

  • Alginates / chemistry
  • Animals
  • Glucuronic Acid / chemistry
  • Growth Hormone / administration & dosage
  • Growth Hormone / pharmacology
  • Growth Hormone / therapeutic use*
  • Hexuronic Acids / chemistry
  • Hydrogels / chemistry
  • Myocardial Infarction / drug therapy*
  • Myocytes, Cardiac / drug effects
  • Rats
  • Rats, Wistar
  • Tissue Scaffolds / chemistry
  • Ventricular Remodeling*


  • Alginates
  • Hexuronic Acids
  • Hydrogels
  • Glucuronic Acid
  • Growth Hormone