Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology

Clin Vaccine Immunol. 2015 Oct;22(10):1130-2. doi: 10.1128/CVI.00390-15. Epub 2015 Aug 19.

Abstract

This study investigated the comparative accuracy of a recombinant 56-kDa type-specific antigen-based rapid diagnostic test (RDT) for scrub typhus for the detection of IgM antibodies by using conventional serology in well-characterized serum samples from undifferentiated febrile illness patients. The RDT showed high specificity and promising comparative accuracy, with 82% sensitivity and 98% specificity for samples defined positive at an IgM indirect immunofluorescence assay positivity cutoff titer of ≥1:1,600 versus 92% and 95% at ≥1:6,400, respectively.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / blood
  • Antigens, Bacterial / immunology
  • Chromatography, Affinity / standards*
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Immunoglobulin M / blood*
  • Male
  • Orientia tsutsugamushi / immunology*
  • Reagent Kits, Diagnostic
  • Scrub Typhus / diagnosis*
  • Scrub Typhus / immunology
  • Sensitivity and Specificity
  • Serologic Tests / standards*

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Immunoglobulin M
  • Reagent Kits, Diagnostic