One-Step Multiplex PCR Assay for Differentiating Proposed New Species "Clostridium neonatale" from Closely Related Species

J Clin Microbiol. 2015 Nov;53(11):3621-3. doi: 10.1128/JCM.01404-15. Epub 2015 Aug 19.

Abstract

"Clostridium neonatale" sp. nov., previously involved in an outbreak of neonatal necrotizing enterocolitis, was recently proposed as a new species of the Clostridium genus sensu stricto. We developed a one-step multiplex colony PCR for C. neonatale identification and investigated C. neonatale intestinal colonization frequency in healthy preterm neonates.

MeSH terms

  • Canada / epidemiology
  • Clostridium / classification*
  • Clostridium / genetics
  • Clostridium / isolation & purification
  • Cross Infection / epidemiology*
  • Cross Infection / microbiology
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • Disease Outbreaks
  • Enterocolitis, Necrotizing / diagnosis*
  • Enterocolitis, Necrotizing / epidemiology*
  • Enterocolitis, Necrotizing / microbiology
  • Feces / microbiology
  • Humans
  • Infant, Newborn
  • Intensive Care Units, Neonatal
  • Intestines / microbiology
  • Multiplex Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 16S / genetics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

  • DNA Primers
  • DNA, Bacterial
  • RNA, Ribosomal, 16S